show Abstracthide AbstractThe goal of the study is to determine the secondary structure of telomerase RNA in P. patens in vivo (using DMS-Seq) or in vitro (using SHAPE-Seq). cDNA with induced mutations was generated with TGIRT-II or Superscript-II in the presence of MnCl2 (SHAPE-Seq). Samples for sequencing were prepared in biological triplicates.